Neotrium | Bio-Architecture Report™

Section 0 — Genetic Risk Score · Auditable Breakdown

How We Got 56 / 100

METHODOLOGY: WEIGHTED SUM × SEVERITY

DNA Trait Flagged	Severity	Weight	Points
9p21.3 CAD Locus — Quintuple Homozygous Risk	HIGH	×3	3
Neuro-Autoimmune Stack ($FOXP3+$CTLA4+$IRF5+$SH2B3+$HLA-DQ8)	HIGH	×3	3
Active Immune Dysregulation (Lymphocytes 49.8% + $FOXP3 TT)	HIGH	×3	3
Elevated LDL 111 mg/dL (vs 9p21.3 risk floor <100)	HIGH	×3	3
Breast Cancer Polygenic 5-SNP Stack ($FGFR2, $TOX3, $MAP3K1)	HIGH	×3	3
Methylation 4-Gene Failure ($MTHFR+$MTR+$MTRR+$SLC19A1)	MED	×2	2
$PNPLA3 NAFLD Risk (CG Het)	MED	×2	2
$COL1A1 Connective Tissue Vulnerability (ACL + Stress Fracture)	MED	×2	2
$VDR Quadruple Variant — Higher Vit D Requirement	MED	×2	2
Higher Vit A Requirement (CircleDNA confirmed)	MED	×2	2
Higher Vit E Requirement	MED	×2	2
Higher Selenium Requirement	MED	×2	2
Higher CoQ10 Requirement	MED	×2	2
Higher Iodine Requirement	MED	×2	2
$CDKAL1 AA — Beta-Cell Insulin Vulnerability	MED	×2	2
Weight Regain Velocity Elevated	MED	×2	2
$ESR1 Estrogen Receptor Double Homozygous (Bone)	MED	×2	2
$CETP rs708272 AA — Genetically Lower HDL Ceiling	MED	×2	2
Lactose Non-Persistence ($LCT/$MCM6 GG+CC)	LOW	×1	1
$CYP1A2 Slow Caffeine Clearance	LOW	×1	1
$ADORA2A Caffeine Anxiety Amplification	LOW	×1	1
$ADH1B Slow Alcohol Metabolism	LOW	×1	1
Super-Taster Phenotype	LOW	×1	1
Spice Sensitivity Heightened	LOW	×1	1
Photoaging Risk High	LOW	×1	1
Acne Risk High	LOW	×1	1
Insomniac Phenotype (CircleDNA)	LOW	×1	1
Fatigue Resistance Below Average	LOW	×1	1
Recovery Efficiency Lower	LOW	×1	1
TOTAL — Risk Band: Moderate-High (56–80)			56 / 100

Reading the score: 0–30 = maintenance only. 31–55 = preventive action. 56–80 = intervention critical (current band). 81–100 = aggressive intervention. Mariam sits at the lower edge of the intervention band — meaning the protocols below are not optional optimization; they are the difference between expression and prevention of identifiable family disease patterns.

How Your Body Systems Connect — The Main Conflict

The Pattern: Your body has two conflicting forces. Your cognitive engine ($BDNF Val/Val + $COMT balanced) is genetically elite — designed for peak performance and elite memory. But your methylation machinery is broken at four genes ($MTHFR + $MTR + $MTRR + $SLC19A1) — meaning the fuel that powers that elite engine is blocked from being made. At the same time, your immune brake pedal ($FOXP3 TT) is genetically weak — and your blood now shows lymphocytes at 49.8% (above 40%), meaning that brake is currently failing. Your father developed Parkinson's, your brother developed MS — both linked to the same broken pathway. You have been handed an early warning your family did not have. The protocols below convert that warning into prevention.

The Engine

Metabolic Fuel

Endurance Engine, Blocked Fuel

Built for sustained output (high VO2, lactate clearance, $ACE hybrid) — but $MTHFR homozygous + $SLC19A1 homozygous mean folate cannot reach the cells that power it.

Power BuildLOW (No Sprint)

Endurance CapacityHIGH

The Chassis

Joints / Bones / Skin

Fragile Frame

$COL1A1 collagen variant + $ESR1 double homozygous + bone density vulnerability = ACL injury risk and stress fracture risk both elevated. The chassis cannot tolerate impact volume.

ACL RiskHIGHER

Stress FractureELEVATED

The Filter

Immune / Detox

Brake Pedal Failing

$FOXP3 TT homozygous + 4 other autoimmune-risk genes = your immune brake pedal is genetically weak. Blood lymphocytes at 49.8% confirm it's already running hot. Brother's MS at 36 came through this same wiring.

Treg FunctionWEAKENED

Lymphocytes (Blood)49.8% ↑

Section I — Your Diet & Metabolism

How Your Body Handles Food

Trait: Healthy Balance + Liver Lipid Sentinel ($PNPLA3)

What This Means

Your body handles fats and carbs normally — you're a "Healthy Balance" diet type. But you have one liver gene ($PNPLA3) that turns fructose and alcohol directly into liver fat. Your blood right now shows the liver is clean (GGT 11, ALT 14) — the protocol is working before it even started. This is your job to protect.

Critical Rules

Zero alcohol — permanent. $ADH1B + $PNPLA3 = double hit on liver.
Zero gluten — permanent. Brother's MS came through this gateway.
Zero dairy — both lactase genes confirm intolerance.
Berries only for fruit (skip juice, mango, tropical).

Your Daily Food Breakdown

Daily Calorie Goal: 2,160 kcal (workout) | 1,950 kcal (rest)

Protein 30% — 162g daily

Animal-source preferred — methylation can't compensate for plant amino acid gaps.

Healthy Fats 35% — 84g daily

Olive oil, avocado, fatty fish. Minimal seed oils. No saturated overload.

Clean Carbs 35% — 190g daily

Sweet potato, quinoa, gluten-free oats, berries. Skip refined flour entirely.

Daily Calorie Adjustments

Rest Days 1,950kcal

Workout Days 2,160kcal

Calculated from BMR 1,394 × activity multiplier. Floor: never below 1,700 kcal (RED-S threshold).

Monthly Cycle Energy Rhythms

FEMALE HORMONE SCHEDULE

Cycle Phase	Food Adjustments	Best Type of Workout	Fasting Rule
Post-Period (Follicular)	Slightly more clean carbs (sweet potato, oats)	Strength + tempo cardio	14 hours
Mid-Cycle (Ovulatory)	Normal eating + extra leafy greens	Peak endurance window	14 hours
Pre-Period (Luteal)	Slightly more dark chocolate + Mg foods	Lighter cardio / Pilates	12 hours
During Period (Menstrual)	Anti-inflammatory: salmon, turmeric, bone broth	Yoga + Walking only	No Fasting

GREEN LIST — Eat Daily

• Wild-caught salmon, sardines, mackerel (3–4×/week)
• Pasture-raised eggs (3–4 daily) — choline for methylation
• Cruciferous vegetables: broccoli, cauliflower, kale (daily, NRF2)
• Leafy greens: spinach, arugula, rocket — natural folate
• Berries: blueberry, raspberry — only safe fructose
• Olive oil (extra virgin) — primary fat source
• Avocado — daily
• Bone broth (3–4×/week) — gut lining repair
• 2 Brazil nuts daily — selenium thyroid support
• Lactose-free protein: hemp, pea, egg white
• Fermented foods (kimchi, sauerkraut — DAIRY-FREE)
• Sweet potato, quinoa, gluten-free oats

RED LIST — Permanent Elimination

• All gluten — wheat, barley, rye, spelt (MS family pathway)
• All dairy — both lactase genes confirm intolerance
• All alcohol — $ADH1B slow + $PNPLA3 liver
• Fruit juice / liquid fructose — PNPLA3 liver fat
• Tropical fruit (mango, pineapple) — high fructose load
• Caffeine after 9:30 AM — $CYP1A2 + $ADORA2A double hit
• Seed oils: canola, sunflower, soybean, corn (inflammatory)
• Folic acid (in fortified foods) — actively harmful for MTHFR
• High-mercury fish (tuna, swordfish) — neurotoxin
• High-spice foods — super-taster amplification
• Ultra-processed packaged foods

Section II — Your Weekly Workout Plan

How to Exercise Right for You

Your DNA shows endurance + strength dominant, low explosive power, high VO2 efficiency, above-average lactate clearance — but below-average fatigue resistance and recovery. You are built for sustained moderate output, not sprint cliffs. $COL1A1 + ACL risk + stress fracture elevated means impact volume must be controlled.

Best Time to Exercise

06:30 – 09:00 AM

Your Morning Lark chronotype + cortisol peak + caffeine window before 9:30 AM cutoff = peak performance window. Afternoon training conflicts with $CYP1A2 caffeine residue and lower fatigue resistance.

⚠️ Dizzy During Workout? — Pre-Session Protocol

Your low-BP profile ($NOS3 protective) + uric acid 2.4 (low) + BUN/Cr 25 (mild dehydration) = exercise-induced hypotension is the most likely cause — not iron deficiency.

• 60–90 min before: protein + carb meal
• 30 min before: 500ml water + ½ tsp sea salt
• 10 min warm-up minimum (gradual BP ramp)
• Sip water continuously through session

Your Ideal Weekly Schedule

2× Strength Training Compound lifts, 6–10 reps

2× Steady-State Cardio 40–60 min, swim/cycle preferred

1× Yoga / Pilates Mobility + nervous system reset

Daily Walk 30 min minimum (Parkinson's prevention)

2× Full Rest Days Mandatory — recovery genetically slow

ACL PROTECTION PROTOCOL — Non-Negotiable

Take 15g Collagen Peptides + 1g Vitamin C 30–45 minutes before EVERY training session. This activates collagen synthesis at the ligament during the workout window. Combined with 10-min dynamic warm-up. No exceptions.

Section III — Strategic Supplement Stack

RANK 0 — Life Critical (Address Methylation + Family History)

START THIS WEEK

Methylcobalamin (Sublingual) 5,000 mcg | Morning

$MTHFR A1298C homozygous + $SLC19A1 homozygous bypass. NEVER cyanocobalamin.

L-Methylfolate (5-MTHF) 1,000 mcg | Morning

Active folate. NEVER folic acid (toxic for her profile — cannot enter cells).

TMG (Trimethylglycine) 2,000 mg | Morning

Backup methylation donor — bypasses the broken MTHFR pathway.

P5P (Active B6) 50 mg | Morning

Homocysteine clearance cofactor. Critical for neuroprotection.

Riboflavin-5-Phosphate (Active B2) 25 mg | Morning

MTHFR enzyme cofactor. Activates the genetic backup pathway.

Vitamin D3 + K2 MK-7 8,000 IU + 200 mcg | With Lunch

$VDR quadruple variant + brother's MS = aggressive D target 85–100 ng/mL.

RANK 1 — Structural & Daily Foundation

Omega-3 (High EPA, TG-form) 3,000 mg | With Lunch

$FADS1 triple het = poor plant ALA conversion. Marine source mandatory. Myelin support.

CoQ10 (Ubiquinol) 300 mg | With Lunch

Higher Need (CircleDNA). Mitochondrial support — Parkinson's prevention pathway.

Vitamin A (Retinol) 5,000 IU | With Breakfast

Higher Need + Skin Photoaging High. Immune + skin barrier.

Vitamin E (Mixed Tocopherols) 400 IU | With Largest Meal

Higher Need. Antioxidant defense + cardiovascular protection.

Selenium (or 2 Brazil nuts) 200 mcg | Morning

Higher Need. Thyroid T4→T3 conversion + autoimmune support.

Iodine (Kelp / KI) 200 mcg | Morning

Higher Need + TSH high-normal (2.59) + thyroid autoimmune risk.

Magnesium Glycinate 400 mg | 30 min before bed

Sleep onset (Insomniac phenotype) + recovery + cardiovascular.

Collagen Peptides + Vit C 15g + 1g | Pre-workout

$COL1A1 + ACL Higher Risk. Take 30–45 min before EVERY session.

Zinc + Copper 30 mg + 2 mg | Evening

Connective tissue, skin (acne risk), immune. Take away from copper foods.

RANK 2 — Neuroprotective & Gut-Brain Defense

N-Acetyl Cysteine (NAC) 600 mg ×2 | Midday + Evening

Glutathione precursor. Direct neuroprotection (Parkinson's pathway).

Alpha-Lipoic Acid 600 mg | With Lunch

Crosses blood-brain barrier. Mitochondrial antioxidant.

Curcumin (Liposomal/Meriva) 500 mg | With Lunch

Anti-neuroinflammatory. Crosses blood-brain barrier.

Lion's Mane Mushroom 1,000 mg | Evening

Stimulates Nerve Growth Factor (NGF). Long-term neural reserve.

L-Glutamine 5g | Morning, empty stomach

Gut lining repair. Critical for HLA-DQ8 + autoimmune profile.

Berberine HCl 500 mg ×2 | With Meals

$PNPLA3 liver + $CDKAL1 + Weight Regain elevated. Insulin sensitizer.

DO NOT TAKE — Currently Contraindicated

✅ Iron — NOW ACTIVE (Apr 2026) Ferritin 10.80 ng/mL = DEFICIENT

Thorne Ferrous Bisglycinate 25mg, every OTHER day, empty stomach + 500mg Vit C, AWAY from coffee/calcium/zinc. Re-test ferritin in 90 days. Target 50–100 ng/mL.

❌ Folic Acid (Synthetic) Cannot bypass $SLC19A1 TT

Synthetic folic acid blocks folate receptors and worsens MTHFR pathway. Check all multivitamins and fortified foods.

❌ Cyanocobalamin B12 Methylation cannot convert

Cheap B12 form requires methylation she doesn't have. Methylcobalamin only.

❌ High-Dose Soy Isoflavones $ESR1 double homozygous

Phytoestrogens may interact with estrogen receptor variants + breast polygenic SNPs.

Section IV — Blood Work & Health Targets

Tracking Your Internal Health — Nov 2025 + 27 Apr 2026 Panels

2 PANELS · 3 FLAGS RESOLVED

Health Marker	Where You Are Now	Where We Want You	Why It Matters
HbA1c	5.0% ✓	< 5.4%	Excellent — PNPLA3 risk currently suppressed
Triglycerides	40 ✓ ELITE	< 80	Exceptional — fructose discipline working
LDL Cholesterol	111 ↑	< 100	9p21.3 quintuple makes <100 hard target
HDL Cholesterol	69 ↓	> 77	$CETP AA = genetic ceiling on HDL
Vitamin D (25-OH)	71.4 ng/mL	85–100 ng/mL	$VDR variants + brother MS = aggressive target
Vitamin B12	586 pg/mL	700–900	Suboptimal for $MTHFR — increase methyl-B
Lymphocytes % ↻ RETESTED	32.14% ✓	16–46%	RESOLVED — was 49.8% Nov, immune fire settled
MPV (Platelet Volume) ↻ RETESTED	10.3 fL ✓	7.9–10.8	RESOLVED — was 12.8 Nov, no longer pro-thrombotic
CA 15-3	34.48	< 35	Borderline — re-test in 90 days
Liver (ALT/GGT)	14 / 11 ✓	< 25 / < 30	PNPLA3 risk currently zero — protect
Ferritin ⚠ NEW	10.80 ng/mL ↓↓	50–100	DEFICIENT — true iron deficiency without anemia. Start Thorne iron NOW.
Iron / TS	108 / 28%	80–150 / 25–40%	Normal in circulation but ferritin (storage) is empty
Hemoglobin ↻ RETESTED	13.8 g/dL ✓	11.5–16	Normal — body protecting RBC production despite low ferritin
tTG-IgA (Celiac) ⚠ NEW	< 0.50 ✓	< 15 (Negative)	Negative — no active celiac. $HLA-DQ8 predisposition still present
tTG-IgG (Celiac) ⚠ NEW	< 0.8 ✓	< 15 (Negative)	Negative — celiac ruled out (assuming gluten in diet pre-test)
Eosinophils % ⚠ NEW	0.46% ↓	0.5–7	Mildly low — possibly cortisol/stress-driven, monitor
TSH	2.59	1.0–2.0	High-normal — autoimmune thyroid screen

27 April 2026 Retest — What Changed

3 NEW FINDINGS

🔴 NEW DEFICIENCY

Ferritin: 10.80 ng/mL

True iron deficiency without anemia confirmed. Hemoglobin still normal (13.8) but storage tank is empty. This is the actual cause of workout dizziness — not RED-S, not low BP.

✓ START Thorne Iron 25mg
✓ Every OTHER day, empty stomach
✓ With 500mg Vit C
✓ AWAY from coffee/tea/calcium
✓ Re-test ferritin in 90 days

✅ FLAGS RESOLVED

Lymphocytes 32.1% · MPV 10.3

November showed lymphocytes 49.8% + MPV 12.8 (concerning). April shows both fully normal. Active immune dysregulation has settled. Likely transient (subclinical viral / stress).

✓ FOXP3 fire NOT actively burning
✓ Genetic risk remains, but not flaring
✓ Continue prevention protocol
✓ Re-monitor 6 months

✅ CELIAC: NEGATIVE

tTG-IgA <0.50 · tTG-IgG <0.8

No active celiac. BUT $HLA-DQ8 + brother's MS pathway = stay gluten-free preventively. Negative tTG depends on having eaten gluten pre-test.

✓ Continue gluten-free lifestyle
✓ Reason now = prevention, not damage
✓ Non-celiac gluten sensitivity still possible
✓ Ask for tTG6 (neuro-celiac) next

Why ferritin was missed in November: The Nov panel measured serum iron (108) which looked normal — but didn't include ferritin (storage). Iron deficiency without anemia hides in normal serum iron. The doctor's clinical instinct to start iron was correct. The supplement product on hand (Thorne Ferrous Bisglycinate) is the ideal form. Verdict: Doctor was right on the action. Now the deficiency is confirmed and the protocol is correct.

Still Pending Tests — Order Next

9 REMAINING · 3 DONE

CRITICAL — METHYLATION

Homocysteine

$MTHFR validation — target < 7 µmol/L

CRITICAL — IMMUNE

Anti-TPO + Anti-TG

Hashimoto's screen — TSH 2.59 + FOXP3 TT

✅ DONE — APR 2026

tTG-IgA + tTG-IgG (Celiac)

Negative — celiac ruled out. Still order tTG6 next visit.

CRITICAL — IMMUNE

ANA + Lymphocyte Subset (CD4/CD8/Treg)

Lupus + Treg count (the FOXP3 measurement)

CRITICAL — CARDIO

ApoB + Lp(a)

9p21.3 quintuple — particle count, not LDL

CRITICAL — METABOLIC

Fasting Insulin + HOMA-IR

$CDKAL1 hidden risk — HbA1c misses early IR

IMPORTANT

hs-CRP

Inflammation baseline + unlocks PhenoAge calc

IMPORTANT

Anti-MOG + Anti-MAG

Myelin antibodies — MS family screen

IMPORTANT

EBV Panel (VCA IgG/IgM, EBNA)

Rule out reactivated EBV driving lymphocytes

✅ DONE — APR 2026

Ferritin: 10.80 ng/mL

DEFICIENT — supplement now. B12 active still pending.

MONITOR

CA 15-3 Re-test

Re-check in 90 days. Trend > single value

MONITOR

APOE Clinical Genotype

rs429358 not in panel — decisive cardio unknown

Section V — Gut Health & Digestion

Inferred from DNA + blood (no microbiome panel uploaded). Your gut is the front door for the autoimmune cascade. With $HLA-DQ8 (gluten gateway gene) + $FOXP3 weak Tregs, every gluten exposure cracks the door open. Your blood lymphocytes at 49.8% suggest the door is currently ajar.

Gluten Cross-Reactivity

Status: CRITICAL — Lifelong Cutoff Required

$HLA-DQ8 + brother's MS = molecular mimicry pathway. Even oats may cross-react in ~10%.

Lactose Tolerance

Status: Genetically Intolerant

$LCT + $MCM6 both confirm. Permanent dairy elimination — non-negotiable.

Gut Lining Integrity

Status: Repair Mandate

L-Glutamine 5g + Zinc Carnosine + bone broth + fermented (DAIRY-FREE) foods.

Inflammation Baseline

Status: Likely Low ($IL6 GG)

IL6 protective variant. Confirm with hs-CRP test (currently pending).

Section VI — The Paradox Vault (Glitches & Unlocks)

WHAT HAPPENS

Four genes ($MTHFR + $MTR + $MTRR + $SLC19A1) all carry risk variants. $SLC19A1 is homozygous — meaning folate physically cannot enter your cells through the normal door. Standard B vitamins do nothing. Your body cannot make the brain chemicals (serotonin, dopamine, melatonin) without methylation. It also cannot repair myelin (the MS gene). This is rare — under 3% of the population has all four hits.

THE UNLOCK

Bypass the broken machinery: Sublingual methylcobalamin 5,000 mcg + L-methylfolate 1,000 mcg + TMG 2g + P5P + B2 active forms. NEVER folic acid. NEVER cyanocobalamin. Test homocysteine to confirm — target < 7 µmol/L. This is life-support, not optimization.

WHAT HAPPENS

Quadruple hit on caffeine. $CYP1A2 = slow clearance (12–16 hrs). $ADORA2A = anxiety amplifying receptor. CircleDNA confirms High Sensitivity. Result: a 2 PM coffee is still chemically active at midnight. You feel "wired but tired," anxious, and you don't sleep. This is also why your "Insomniac" CircleDNA flag exists — it's caffeine-mediated, not genetic insomnia.

THE UNLOCK

Hard 9:30 AM cutoff — no exceptions. Replace afternoon coffee with matcha (L-theanine buffered) before noon, then water/herbal-only. Always pair morning coffee with food (oat milk + fat slows the spike). 3 nights of consistency = insomnia phenotype resolves.

WHAT HAPPENS

Five genes weaken your immune brake pedal: $FOXP3 TT (homozygous on X — both copies hit), $CTLA4, $SH2B3, $IRF5, $HLA-DQ8. Your brother developed MS at 36 through this exact wiring. Your blood now shows lymphocytes at 49.8% (above 40%) — meaning your immune system is currently activated. The paradox: you have $IL6 protective genotype (low chronic inflammation), so this won't show on standard CRP. The damage is silent.

THE UNLOCK

Order the autoimmune panel: Anti-TPO/TG, ANA, tTG-IgA + tTG6 (neurological gluten antibody), Anti-MOG/MAG (myelin antibodies), Lymphocyte subset with Treg count. Eliminate gluten permanently. Methylation correction (Rank 0 stack). Vitamin D to 85–100 ng/mL. EBV panel to rule out viral driver. Establish neurologist baseline NOW while healthy.

WHAT HAPPENS

Five SNPs at the 9p21.3 locus — the strongest cardiovascular gene region ever discovered — are ALL homozygous risk. (rs4977574, rs1333049, rs10757278, rs10757274, rs1333042). Population frequency < 2%. Mechanism: smooth muscle cell proliferation in artery walls — structural CAD risk. Combined with current LDL 111 + low HDL 69 + MPV 12.8 (sticky platelets) = a real, measurable risk profile that needs active management.

THE UNLOCK

Endurance training directly suppresses 9p21.3 expression — your prescribed cardio plan IS your therapy. Omega-3 3g/day reduces platelet aggregation. ApoB < 80 target (not standard LDL). Discuss low-dose aspirin with cardiologist given MPV elevation. Annual ApoB + Lp(a) tracking. Discuss APOE clinical test. Berberine for insulin sensitivity.